review paper for rice sheath blight 2016 2019

solani AG1-IA and AG1-IB can infect some asterids and soybean ( Hane et al., 2014), lead to rice sheath blight (ShB), and has been the most important factor in limiting rice production over the past two decades ( Chen et al., 2012 Susheela and Reddy, 2013 Arakawa and Inagaki, 2014). One of these, AG1, can be further subdivided into four intraspecific groups (ISGs), AG1-IA∼AG1-ID. Rhizoctonia solani is a devastating soil-borne fungus which is classified into fourteen reproductively incompatible anastomosis groups (AGs). During the cropping period, the plant may be attacked by various pathogens, including Pyricularia oryzae, Bipolaris oryzae, Rhizoctonia solani, Fusarium fujikuroi, and Xanthomonas oryzae pv. According to data from the FAO (Food and Agriculture Organization of the United Nations), the global yield of rice is nearly 782 million metric tons per year.

Paddy rice ( Oryza sativa), which is one of the world’s main food crops, takes up nearly 11% of the arable land worldwide, in addition to being the food that the largest proportion of people eat as their primary food ( FAOSTAT, 2019). These results indicated that the two molecular detection protocols were suitable for the field diagnosis of ShB for all asymptomatic and symptomatic rice samples. In addition, all the samples of infected rice yielded positive Rs detection results when subjected to TaqMan probe-based real-time PCR and SYBR green-based real-time PCR (SMS RS1-F/SMS RS1-R) tests in which automatic magnetic bead-based DNA extraction was performed. The results indicated that all of the developed protocols are suitable for use in detecting ShB. Moreover, intraday and interday assays were performed to evaluate the reproducibility of the detection methods developed.

The specificity and sensitivity of a novel SMS RS1-F/SMS RS1-R primer set and a ITS1/GMRS-3 reference primer set were tested, while four different extraction protocols for ShB were developed. In this study, a reagent for the rapid extraction of this pathogen was developed for on-site detection. To prevent outbreaks of ShB and enhance the sustainability of rice production, it is critical to develop a rapid ShB detection method for specific, fast, and on-site disease management. Rhizoctonia solani (Rs), a soil-borne fungal pathogen, can result in rice sheath blight (ShB), which causes yield loss.

Review paper for rice sheath blight 2016 2019